Neutrophil and EV Workshop
This workshop will be hands-on training to purify and analyze extracellular vesicles (EVs) as well as studying and manipulating neutrophils and perhaps other innate immune cells such as monocytes. Our goal for this event is to promote education on current techniques to 1) study and manipulate innate immune cells and 2) study and analyze extracellular vesicles. The activation state of innate immune cells has been implicated in most diseases and EVs play an important role in coordinating immune cell behavior and can serve as biomarkers of disease.
The dates of the workshop are November 7th to 10th. It will be located in our lab space at 2015 Uppergate Drive.
For questions, please reach out to Brian Dobosh (bdobosh@emory.edu) and Rabin Tirouvanziam (tirouvanziam@emory.edu).
- This workshop will focus on purifying and analyzing EVs as well as studying and manipulating neutrophils. The dates of the workshop are November 7th to 10th. Please note that you will be expected to attend each day of the hands-on training. We expect the training to last the entirety of each day. Certificates for BSL2 and bloodborne pathogen training will be required per EHSO guidelines.
For questions please reach out to Brian Dobosh (bdobosh@emory.edu) and Rabin Tirouvanziam (tirouvanziam@emory.edu)
Topics:
This list is likely to be expanded:
- Purification of EVs from human sputum and BAL
- Determining concentration and size of EVs via nanoparticle tracking analysis (NTA)
- Analysis of EV cell-of-origin by NTA and nanoflow cytometry
- Transfection of neutrophils with nucleic acids (plasmids, mRNA, siRNA)
- Handling of an airway in-vitro transmigration model (https://www.sciencedirect.com/science/article/pii/S2666166721005980)
Cost:
$0 There is no registration fee.
We will offer lunch during the day, but we cannot subsidize flights or accommodations. Once everyone has registered we will send out a mass email so that there can be potential coordination of lodging and flights (if possible). We will likely also have a lab outing one of the evenings, which you will be included in.
Register here for the workshop
Information on the associated symposium here
Agenda:
We will be starting at 10 AM EST each day to accommodate those that like to sleep in or are experiencing time changes. We expect to be done by 4 PM each day. We have some activities planned for the early evening time that are obviously completely optional – details still pending but we are thinking of perhaps bowling, going to an arcade or minigolf.
Weather in November may be cold for some and warm for others!
We will be starting at 10 AM EST each day to accommodate those that like to sleep in or are experiencing time changes. We expect to be done by 4 PM each day. We have some activities planned for the early evening time that are obviously completely optional – details still pending but we are thinking of perhaps bowling, going to an arcade or minigolf.
Weather in November may be cold for some and warm for others!
Monday, November 7th
AM: Meet and Greet
Overview of the plan, review of the protocols and theory for the workshop (~2 hours)
Lunch
PM: Purification of EVs from sputum and quantification of EVs using Nanosight (~2 hours)
Tuesday, November 8th
AM: Purification of neutrophils and monocytes from primary human blood (~3 hours)
Lunch
PM: Electroporation of cells with plasmids and use of the transmigration model (~2 hours)
Wednesday, November 9th
AM: Purification of transmigrated cells (~2 hours)
Staining cells for flow cytometry
Lunch
PM: Staining and running EV samples to analyze cell of origin of EVs using flow cytometry (~3 hours)
Thursday, November 10th
AM: Running of stained cells for flow cytometry and analysis of flow cytometry data (~2 hours)
PM: Wrap-up and full-lab outing at brewery in Atlanta
